產(chǎn)品詳情
簡(jiǎn)單介紹：
重組人甲狀旁腺素1 - 84、15 n穩(wěn)定同位素標(biāo)記蛋白與其它公司提供的重組蛋白不同，rHuPTH1-84, 15N蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組人甲狀旁腺素1 - 84、15 n穩(wěn)定同位素標(biāo)記蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組人甲狀旁腺素1 - 84、15 n穩(wěn)定同位素標(biāo)記蛋白
| Synonyms | Parathormone, Parathyrin |
| Species | Human |
| Accession | P01270 |
| GeneID | 5741 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人甲狀旁腺素1 - 84、15 n穩(wěn)定同位素標(biāo)記蛋白Approximately 9551 Da, a single non-glycosylated polypeptide chain containing 84 amino acids. 15N stable isotope labeled. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人甲狀旁腺素1 - 84、15 n穩(wěn)定同位素標(biāo)記蛋白Fully biologically active when compared to standard. The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組人甲狀旁腺素1 - 84、15 n穩(wěn)定同位素標(biāo)記蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuPTH1-84, 15N as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人甲狀旁腺素1 - 84、15 n穩(wěn)定同位素標(biāo)記蛋白 | |
| Reference |
1. Potts, J.T. 2005. J Endocrinol, 187: 311-25. 2. Misiorowski, W. 2011. Endokrynol Pol, 62: 73-8. 3. Scillitani, A., V. Guarnieri, C. Battista, et al. 2011. J Endocrinol Invest, 34: 23-6. |
| Background | Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. PTH is a critical hormone in the regulation of Ca2+ homeostasis. PTH is secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low. |
