產品詳情
簡單介紹：
重組人類SOX2-TAT蛋白與其它公司提供的重組蛋白不同，rHuSOX2-TAT蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類SOX2-TAT蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類SOX2-TAT蛋白
| Synonyms | SOX2-TAT |
| Species | Human |
| Accession | P48431 |
| GeneID | 6657 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類SOX2-TAT蛋白Approximately 36.0 kDa, a single non-glycosylated polypeptide chain containing 330 amino acids, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR). |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR |
| Purity | 重組人類SOX2-TAT蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data Not Available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, with 5% Trehalose. |
| Endotoxin | Less than 1 EU/μg of rHuSOX2-TAT as determined by LAL method. |
| Reconstitution | 重組人類SOX2-TAT蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類SOX2-TAT蛋白 | |
| SDS-PAGE |
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| Reference |
1. M Stevanovic, O Zuffardi, J Collignon, et al. 1994. Mamm Genome, 5: 640-2. 2. J Fantes, NK Ragge, SA Lynch, et al. 2003. Nat Genet, 33: 461-3. 3. A Rizzino. 2009. Wiley Interdiscip Rev Syst Biol Med, 1: 228-36. 4. K Takahashi, K Tanabe, M Ohnuki, et al. 2007. Cell, 131: 861-72. |
| Background | Sox2, also named SRY (sex determining region Y)-box 2, is belonging to the Sox family and it is encoded by the Sox gene in human. This protein family shares highly conserved DNA binding domains known as HMG (High-mobility group) box domains containing approximately 80 amino acids. Sox2 plays a role in maintenance of embryonic and neural stem cells and holds great promise in research involving induced pluripotency, an emerging and very promising field of regenerative medicine. Mature human Sox2 shares 100 amino acid sequence identity with murine and rat Sox2. Recombinant human Sox2-TAT expressed in E. coli is a 36 kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR). |
