產(chǎn)品詳情
簡單介紹：
重組大鼠嗜中性粒細(xì)胞活化肽2 / CXCL7蛋白與其它公司提供的重組蛋白不同，rRtNAP-2/CXCL7蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠嗜中性粒細(xì)胞活化肽2 / CXCL7蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠嗜中性粒細(xì)胞活化肽2 / CXCL7蛋白
| Synonyms | CXC Chemokine RTCK1, Chemokine (C-X-C Motif) Ligand 7, Isoform CRA_b, Protein Ppbp. |
| Species | Rat |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 6.8 kDa, a single non-glycosylated polypeptide chain containing 62 amino acids. |
| Quantity | 重組大鼠嗜中性粒細(xì)胞活化肽2 / CXCL7蛋白2μg/10μg/1000μg |
| AA Sequence | IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP TAPMIKKIVK KI |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠嗜中性粒細(xì)胞活化肽2 / CXCL7蛋白Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.1-1.0 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重組大鼠嗜中性粒細(xì)胞活化肽2 / CXCL7蛋白Less than 1 EU/μg of rRtNAP-2/CXCL7 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠嗜中性粒細(xì)胞活化肽2 / CXCL7蛋白 | |
| SDS-PAGE |
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| Reference |
1. Hristov M, Zernecke A, Bidzhekov K, et al. 2007. Circ Res. 100:590-7. 2. Majumdar S, Gonder D, Koutsis B, et al. 1991. J Biol Chem. 266:5785-9. 3. Krijgsveld J, Zaat SA, Meeldijk J, et al. 2000. J Biol Chem. 275:20374-81. 4. Piccardoni P, Evangelista V, Piccoli A, et al. 1996. Thromb Haemost. 76:780-5. |
| Background | CXCL7 is a small cytokine which is also an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein belonging to the CXC chemokine family and it is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant Rat CXCL7 contains 62 amino acids which is a single non-glycosylated polypeptide chain. In addition, The rat CXCL7 shares 58 % and 77 % a.a. sequence identity with human and mouse CXCL7. |
