產品詳情
簡單介紹：
重組體人Interleukin-21蛋白與其它公司提供的重組蛋白不同，rHuIL-21蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組體人Interleukin-21蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組體人Interleukin-21蛋白
| Synonyms | Za11 |
| Species | Human |
| Accession | Q9HBE4 |
| GeneID | 59067 |
| Source | Escherichia coli. |
| Molecular Weight | 重組體人Interleukin-21蛋白Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組體人Interleukin-21蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuIL-21 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組體人Interleukin-21蛋白 | |
| SDS-PAGE |
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| Reference |
1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43. 2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91. 3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264. 4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32. |
| Background | 重組體人Interleukin-21蛋白Human IL-21 is encoded by IL-21 gene located on Chr. 4. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. |
