產品詳情
簡單介紹：
重組人MHC I類多肽相關序列A，His蛋白與其它公司提供的重組蛋白不同，rHuMIC-A, His蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人MHC I類多肽相關序列A，His蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人MHC I類多肽相關序列A，His蛋白
| Species | Human |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 36.9 kDa, a single non-glycosylated polypeptide chain containing 320 amino acids. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | 重組人MHC I類多肽相關序列A，His蛋白MSYYHHHHHH DYDIPTTENL YFQGAMDPEF EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLESGVVLRR TVPPMVNVTR SEASEGNITV TCRASSFYPR NIILTWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC RGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHKLGCFGG |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人MHC I類多肽相關序列A，His蛋白Fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, and 8 M Urea. |
| Endotoxin | Less than 1 EU/μg of rHuMIC-A, His as determined by LAL method. |
| Reconstitution | 重組人MHC I類多肽相關序列A，His蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions, which contain 8 M Urea. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人MHC I類多肽相關序列A，His蛋白 | |
| Reference |
1. Yao Z, Volgger A, Helmberg W, et al. 1999. Eur J Immunogenet, 26: 225-32. 2. Li P, Willie ST, Bauer S, et al. 1999. Immunity, 10: 577-84. 3. Petersdorf EW, Shuler KB, Longton GM, et al. 1999. Immunogenetics, 49: 605-12. 4. Komatsu-Wakui M, Tokunaga K, Ishikawa Y, et al. 1999. Immunogenetics, 49: 620-8. 5. Gambelunghe G, Ghaderi M, Cosentino A, et al. 2000. Diabetologia, 43: 507-14. |
| Background | MIC-A (MHC class I chain-related gene A) is a single-pass type I member protein. It is expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Additionally, MIC-A can be induced by bacterial and viral infections. It shares 85 % amino acid identity with MIC-B and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules. They do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ αβ T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases. |
